Western blot was performed using Anti-PVRIG Polyclonal Antibody (Product # PA5-69630) and a 34 kDa band corresponding to PVRIG was observed across cell lines. Whole cell extracts (30 µg lysate) of Jurkat (Lane1), PANC-1 (Lane 2), IMR-32 (Lane 3), Daudi (Lane 4), A549 (Lane 5), HeLa (Lane 6), K-562 (Lane 7), U-2 OS (Lane 8) and HEL.92.17 (Lane 9) were prepared using RIPA lysis buffer were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # LC2002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.25 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).
Western blot analysis of PVRIG on human brain cells. The sample was probed with a PVRIG polyclonal antibody (Product # PA5-69630) using a primary antibody dilution of 0.2-1.0 µg/mL.
Supplier Page from Thermo Fisher Scientific for PVRIG Antibody